MICB data

• Nucleotide Sequence (Coding sequences)
• Nucleotide Sequence (Genomic sequences)
• Nucleotide Sequence in PIR format
• Nucleotide Sequence in FASTA format

• Peptide Sequence
• Peptide Sequence in PIR format
• Peptide Sequence in FASTA format

• Reference Information

Sequence Formats

FASTA - Sequences in FASTA/Pearson format are represented by two main line types. The first line always begins with a "greater than" (>) sign and contains sequence information. In the files provided, the sequence information contains the name of the HLA allele. The remaining lines contain plain text representing the coding nucleotide sequence. There can be any number of these sequence lines, of any length, to represent the nucleotide sequence.

Example DRB1*0101 in FASTA format:

PIR - The format of sequences in PIR/NBRF format is more complex. The first line of each sequence entry begins with a "greater than" (>) sign. This is immediately followed by a two character sequence type specifier: for the HLA alleles this is "DL", meaning DNA linear. Space four must contain a semi-colon. Beginning in space five is the sequence name or identification code: for HLA alleles this is the official allele name. The second line of each sequence entry contains a brief description, including the sequence length, and an internal checksum for PIR files. The coding nucleic acid sequence begins on the third line. The sequence is free format, but to aid in reading the sequences, the nucleotides have been arranged in blocks of 10 amino acids. The last character is an asterisk (*), and acts as a termination character.

All PIR files have been generated using "ReadSeq", a freely available sequence format conversion program written by D. Gilbert.

Example DRB1*0101 in PIR format.

Enquiries to: hladb@ebi.ac.uk